بررسی تأثیر اسیدسالیسیلیک بر روی علایم بیماری سپتوریای برگی Mycospharella

نویسندگان

1 دانشجوی دورۀ دکتری بیماری‌شناسی گیاهی، دانشگاه تربیت مدرس، تهران، ایران

2 استاد گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس تهران، ایران

3 دانشیار گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس تهران، ایران

4 استادیار پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران

چکیده

در این پژوهش تأثیر اسیدسالیسیلیک بر روی علایم بیماری سپتوریای برگی در ارقام گندم حساس (اترک و کویر) و مقاوم (دریا و زاگرس) در شرایط گلخانه بررسی شد. تیمار اسید سالیسیلیک به دو صورت پاشش بر روی برگ­های گندم و خیساندن بذور در غلظت­های متفاوت این ماده انجام شد. در این بررسی از دو جدایۀ بیماری زای S1 و K8 از قارچ Mycospharella graminicola استفاده شد، که بر اساس نتایج آزمون بیماری زایی جدایۀ S1 بیماری زاتر بود و در آزمون­های بعدی از این جدایه استفاده شد. در آزمون ارزیابی بیماری در تیمار با اسیدسالیسیلیک بر روی گیاه نتایج نشان داد که بین اثر سطوح ارقام و اثر اسیدسالیسیلیک بر روی علایم بیماری اختلاف معنی دار وجود دارد. رقم زاگرس در سطح 4 میلی­مولار اسیدسالیسیلیک با 06/41 درصدکمترین شدت بیماری، و رقم اترک در سطح 5/0 میلی­مولار اسیدسالیسیلیک با 63/81 درصد بیش­ترین شدت بیماری را در این تست نشان دادند. در آزمون تأثیر اسیدسالیسیلیک بر روی علایم بیماری به صورت تیمار خیساندن بذر از میان چهار غلظت اسید سالیسیلیک مورد استفاده در این آزمون (0، 5/0، 1 و 2 میلی­مولار) غلظت 1 میلی­مولار بهترین سطح کنترل کنندگی را داشت. بر اساس نتایج این تحقیق بهترین غلظت پیشنهادی برای پاشش بر روی برگ­های گندم 2 میلی­مولار، و برای تیمار بذور 1 میلی مولار است. 

کلیدواژه‌ها

عنوان مقاله [English]

The evaluation of salicylic acid effect on septoriosis disease by Mycospharella graminicola

نویسندگان [English]

  • J Gholamnejad 1
  • E Mohammadi Goltapeh 2
  • F Sanjarian 3
  • N Safaie 4
  • K.H Razavi 3
چکیده [English]

In this study, the effect of salicylic acid (SA) on response of wheat cultivars (2
susceptible and 2 resistant cvs.) to Septoria was evaluated. The SA was used in two methods;
it was sprayed on the wheat leaves and/or the seeds were drenched in SA solution. Two
isolate of Mycosphaerella graminicola S1 and K8 were used in this study. The pathogenicity
of isolate S1 was more than K8; therefore this isolate was selected for further experiments. In
the test of evaluation disease interaction with SA, Zagrus cultivar showed the least disease
severity (41%) at 4 mM SA level, and Atrak cultivar was showed the most disease severity in
0.5 mM SA level, 81%. Four densities of SA were used for seed treatment (0, 0.5, 1 and
2mM), the maximum controlling of disease was achieved at 1mM level of SA. The results of
this study suggest the best concentration for spraying on wheat leaves is 2 mM of SA, and 1
mM is the best concentration for seed treatment.

کلیدواژه‌ها [English]

  • Mycosphaerella graminicola
  • Salicylic acid
  • Disease evaluation
  • Seed treatment

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